Tchemtchoua V, Atanasova G, Abdelhafid A, Maquet V, Jérôme C, Poumay Y, Colige A. Development of a procedure to simultaneously isolate RNA, DNA, and proteins from characterizing cells invading or cultured on chitosan scaffolds. Anal Biochem. 2009;393:145-147. doi: 10.1016/j.ab.2009.06.013.
This study introduces an ultracentrifugation-based method that enables the concurrent extraction of high-quality RNA, DNA, and proteins from cells cultured on chitosan-containing biomaterial matrices. This approach addresses the challenge of efficiently and quantitatively isolating these macromolecules from cells in close contact with chitosan scaffolds, facilitating comprehensive molecular analyses of cell-scaffold interactions.
Keywords: Chitosan scaffolds, RNA isolation, DNA extraction, protein purification, ultracentrifugation, cell-scaffold interactions