Claverie E, Perini M, Onderwater RCA, Pianezze S, Larcher R, Roosa S, Yada B, Wattiez R. Multiple technology approach based on stable isotope ratio analysis, Fourier transform infrared spectrometry and thermogravimetric analysis to ensure the fungal origin of the chitosan. Molecules. 2023;28:4324. doi: 10.3390/molecules28114324.
This study presents a robust analytical strategy to distinguish between fungal and crustacean-derived chitosan. Researchers employed stable isotope ratio (SIR) analysis, Fourier transform infrared (FTIR) spectrometry, and thermogravimetric analysis (TGA) to identify characteristic markers of chitosan’s origin. Key findings include:
- Stable Isotope Ratio Analysis: Fungal chitosan exhibited distinct δ¹³C, δ¹⁵N, and δ¹⁸O values compared to crustacean chitosan. Specifically, δ¹³C values above -14.2‰ or below -25.1‰, and δ¹⁸O values lower than +25.3‰, were indicative of fungal origin.
- FTIR Spectrometry: Differences in the Amide I and NH₂/Amide II peak areas allowed for differentiation between fungal and crustacean chitosan.
- Thermogravimetric Analysis: Variations in maximum degradation temperatures further supported the discrimination between the two sources.
Combining these methods provides a comprehensive approach to accurately determine the origin of chitosan, ensuring authenticity and compliance in applications where the source is critical.
Keywords: Chitosan origin, stable isotope ratio analysis, FTIR spectrometry, thermogravimetric analysis, fungal chitosan, crustacean chitosan